5H1O
CRISPR-associated protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-31 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 63 |
| Unit cell lengths | 90.385, 90.385, 50.831 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.585 - 1.650 |
| R-factor | 0.1683 |
| Rwork | 0.166 |
| R-free | 0.20520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4es2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.413 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.071 | 0.809 |
| Number of reflections | 28581 | |
| <I/σ(I)> | 12.6 | 3.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 12.1 | 11.5 |
| CC(1/2) | 0.899 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 50mM Ammonium acetate, 85mM Sodium acetate pH 4.6, 23%(w/v) PEG4000. 10%(v/v) Glycerol |
