5H1J

Crystal structure of WD40 repeat domains of Gemin5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 1 21 1
Unit cell lengths	60.258, 123.592, 61.732
Unit cell angles	90.00, 119.27, 90.00

Refinement procedure

Resolution	40.599 - 2.000
R-factor	0.1586
Rwork	0.157
R-free	0.19880
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.911
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.116	0.066	0.504
Number of reflections	53257
<I/σ(I)>	4.6
Completeness [%]	99.9	99.9	99.8
Redundancy	4.8	4.9	4.8
CC(1/2)		0.993	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	0.1M Sodium malonate (pH 7.0), 12% polyethylene glycol (PEG) 3350