5H1A
Crystal structure of an IclR homolog from Microbacterium sp. strain HM58-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2015-11-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.038 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 90.889, 155.375, 83.262 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.600 - 2.100 |
| R-factor | 0.22763 |
| Rwork | 0.227 |
| R-free | 0.24187 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.462 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 69549 | |
| <I/σ(I)> | 24.9 | |
| Completeness [%] | 98.9 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 8 | 293 | 1M (NH4)2HPO4 |
