5H19
EED in complex with PRC2 allosteric inhibitor EED162
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979112 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 50.490, 90.990, 179.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.190 - 1.900 |
R-factor | 0.1786 |
Rwork | 0.177 |
R-free | 0.21540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qxv |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.190 | 1.911 |
High resolution limit [Å] | 1.900 | 1.905 |
Rmerge | 0.134 | |
Number of reflections | 33040 | |
<I/σ(I)> | 11.8 | |
Completeness [%] | 100.0 | |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M Bis-Tris, 0.2 M MgCl2, 20% PEG 3350 |