5H11
Crystal structure of Zebrafish Sec10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2015-12-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9185 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.693, 162.652, 45.572 |
| Unit cell angles | 90.00, 95.55, 90.00 |
Refinement procedure
| Resolution | 46.917 - 2.732 |
| R-factor | 0.2378 |
| Rwork | 0.236 |
| R-free | 0.26700 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.607 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.780 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Number of reflections | 26989 | |
| <I/σ(I)> | 10.7 | 1.5 |
| Completeness [%] | 95.3 | 75.9 |
| Redundancy | 8.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES-Na buffer, 0.57 M K Na tartrate |
