5H0W

Crystal structure of H88F mutated human transthyretin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	I 2 2 2
Unit cell lengths	41.480, 62.820, 85.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.785 - 1.904
R-factor	0.1973
Rwork	0.195
R-free	0.23610
RMSD bond length	0.007
RMSD bond angle	1.119
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.600
High resolution limit [Å]	1.900
Number of reflections	9071
<I/σ(I)>	12.9
Completeness [%]	100.0
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	2.0 M ammonium sulfate, 25% glycerol, 0.1 M MES