Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 75.763, 75.763, 92.804 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.072 - 1.501 |
| R-factor | 0.1843 |
| Rwork | 0.183 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | I-TASSER |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.837 |
| Data reduction software | HKL-2000 (v708c) |
| Data scaling software | HKL-2000 (v708c) |
| Phasing software | PHENIX ((1.10.1_2155)) |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.072 | 1.538 |
| High resolution limit [Å] | 1.501 | 1.501 |
| Rmerge | 0.051 | 0.387 |
| Number of reflections | 45643 | |
| <I/σ(I)> | 33.7 | 1.4 |
| Completeness [%] | 94.6 | 62 |
| Redundancy | 13.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 5mM Tris-HCl(pH 7.0), 0.1M MES(pH 5.0), 30%(v/v) polyethylene glycol 200 |
