5GZU

Crystal Structure of Chitinase ChiW from Paenibacillus sp. str. FPU-7 Reveals a Novel Type of Bacterial Cell-Surface-Expressed Multi-Modular Enzyme Machinery

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	111.981, 127.990, 162.078
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.030
R-factor	0.18456
Rwork	0.182
R-free	0.22581
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1itx
RMSD bond length	0.020
RMSD bond angle	2.011
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.070
High resolution limit [Å]	2.030	2.030
Rmerge	0.058	0.363
Number of reflections	149010
<I/σ(I)>	51.4	6.82
Completeness [%]	99.0	100
Redundancy	7.1	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	(NH4)2HPO4, Na-citrate