5GZH

Endo-beta-1,2-glucanase from Chitinophaga pinensis - ligand free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-28
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 31
Unit cell lengths	90.965, 90.965, 124.086
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.740 - 1.800
R-factor	0.1699
Rwork	0.168
R-free	0.20437
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eu8
RMSD bond length	0.020
RMSD bond angle	1.852
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.098	0.682
Number of reflections	105566
<I/σ(I)>	21.3	2.3
Completeness [%]	99.0	100
Redundancy	5.3	5.6
CC(1/2)		0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M ammonium iodide, 7% PEG 3350