5GZG

Galectin-8 N-terminal domain carbohydrate recognition domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-19
Detector	DECTRIS PILATUS 12M
Wavelength(s)	0.9785
Spacegroup name	P 43 21 2
Unit cell lengths	49.127, 49.127, 159.666
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.470 - 1.997
R-factor	0.2081
Rwork	0.203
R-free	0.25780
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.997
Number of reflections	14014
<I/σ(I)>	14.9
Completeness [%]	99.3
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M Tris pH 8.5, 0.01M NiCl2,20% (W/V) PEG2000 MME