5GZF

Galectin-8 N-terminal domain carbohydrate recognition domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-19
Detector	DECTRIS PILATUS 12M
Wavelength(s)	0.9785
Spacegroup name	P 43 21 2
Unit cell lengths	48.871, 48.871, 159.725
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.408 - 2.002
R-factor	0.1987
Rwork	0.196
R-free	0.21800
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000
Number of reflections	13850
<I/σ(I)>	13.3
Completeness [%]	99.7
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.1M Tris pH 8.5, 0.01M NiCl2, 20% (W/V) PEG 2000 MME