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5GZB

Crystal Structure of Transcription Factor TEAD4 in Complex with M-CAT DNA

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRF BEAMLINE BL17U
Synchrotron siteSSRF
BeamlineBL17U
Temperature [K]100
Detector technologyCCD
Collection date2014-03-30
DetectorRAYONIX MX-225
Wavelength(s)0.97924
Spacegroup nameI 41 2 2
Unit cell lengths118.595, 118.595, 110.258
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution37.500 - 2.704
R-factor0.2318
Rwork0.229
R-free0.25620
Structure solution methodSAD
RMSD bond length0.004
RMSD bond angle0.636
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHENIX
Refinement softwarePHENIX (1.9_1692)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]37.5002.750
High resolution limit [Å]2.7002.700
Number of reflections20397
<I/σ(I)>20.31.5
Completeness [%]99.798.9
Redundancy9.39.1
CC(1/2)0.725
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.52910.6 M Na2HPO4, 0.6 M KH2PO4, 0.1 M HEPES

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