5GZB
Crystal Structure of Transcription Factor TEAD4 in Complex with M-CAT DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-30 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97924 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 118.595, 118.595, 110.258 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.500 - 2.704 |
R-factor | 0.2318 |
Rwork | 0.229 |
R-free | 0.25620 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.636 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.500 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 20397 | |
<I/σ(I)> | 20.3 | 1.5 |
Completeness [%] | 99.7 | 98.9 |
Redundancy | 9.3 | 9.1 |
CC(1/2) | 0.725 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.6 M Na2HPO4, 0.6 M KH2PO4, 0.1 M HEPES |