5GZA

protein O-mannose kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 32
Unit cell lengths	70.550, 70.550, 66.935
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.275 - 2.000
R-factor	0.2032
Rwork	0.197
R-free	0.21830
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.137
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.117	0.108	0.514
Number of reflections	25224
<I/σ(I)>	7.2
Completeness [%]	100.0	99.9	100
Redundancy	7.9	8	7.9
CC(1/2)		0.989	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, ammonium acetate, HEPES