5GZ8
Crystal structure of catalytic domain of Protein O-mannosyl Kinase in ligand-free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2016-06-25 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 81.885, 81.885, 147.745 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.456 - 2.500 |
R-factor | 0.2738 |
Rwork | 0.273 |
R-free | 0.29280 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.583 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.631 | |
Number of reflections | 200696 | |
<I/σ(I)> | 18.1 | 2.9 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 9.8 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl (pH 8.5), 23%(w/v) PEG 3350 |