5GZ7
Crystal Structure of the complex of Ribosome Inactivating Protein with 1,2-ethanediol at 1.95 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2016-06-30 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | H 3 |
Unit cell lengths | 130.197, 130.197, 40.656 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 65.100 - 1.950 |
R-factor | 0.15881 |
Rwork | 0.156 |
R-free | 0.21354 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zz6 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.790 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.100 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 17664 | |
<I/σ(I)> | 0.14 | |
Completeness [%] | 99.3 | 91.7 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 14% PEG 6000, 0.1M Sodium Phosphate |