5GYQ
Putative receptor-binding domain of bat-derived coronavirus HKU9 spike protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.03906 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.752, 35.990, 62.886 |
Unit cell angles | 90.00, 102.72, 90.00 |
Refinement procedure
Resolution | 41.703 - 2.100 |
R-factor | 0.1715 |
Rwork | 0.170 |
R-free | 0.20060 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.820 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 10822 | |
<I/σ(I)> | 34.023 | |
Completeness [%] | 97.1 | 80.7 |
Redundancy | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M sodium citrate tribasic dihydrate pH 7.0, 12% PEG 20000 |