5GYG
Crystal structure of ENZbleach xylanase T28C+T60C mutant with three N-teminal residue truncation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.253, 70.886, 85.254 |
Unit cell angles | 90.00, 90.23, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.740 |
R-factor | 0.1768 |
Rwork | 0.174 |
R-free | 0.22210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gv1 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.379 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.010 | 50.000 | 1.800 |
High resolution limit [Å] | 1.740 | 3.750 | 1.740 |
Rmerge | 0.032 | 0.025 | 0.095 |
Number of reflections | 48098 | ||
<I/σ(I)> | 22.9 | ||
Completeness [%] | 93.2 | 89.1 | 82.1 |
Redundancy | 2.1 | 2.2 | 1.8 |
CC(1/2) | 0.998 | 0.978 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 298 | PEG 3350, 0.1 M tri-sodium acetate pH 4.5, 0.1 M Bis-Tris pH 5.5 |