5GYD
Crystal Structure of Mdm12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 142.592, 219.073, 73.097 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.683 - 3.106 |
| R-factor | 0.2156 |
| Rwork | 0.214 |
| R-free | 0.25350 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.343 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.110 | |
| Number of reflections | 41953 | |
| <I/σ(I)> | 19.9 | |
| Completeness [%] | 99.6 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | Sodium formate, ADA, DTT |
