5GYA

Crystal structure of ENZbleach xylanase V5N+V6N+K7R+K223R+K227R mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-23
Detector	Nonius Kappa CCD
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	42.501, 46.250, 78.204
Unit cell angles	79.57, 83.10, 66.25

Refinement procedure

Resolution	30.000 - 1.930
R-factor	0.1684
Rwork	0.166
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gv1
RMSD bond length	0.011
RMSD bond angle	1.375
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.000
High resolution limit [Å]	1.930	4.150	1.930
Rmerge	0.033	0.027	0.101
Number of reflections	37834
<I/σ(I)>	23.9
Completeness [%]	93.9	98.6	83.2
Redundancy	2.9	3	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5	298	PEG 6000, 0.1 M NaOAc, 0.2 M NaCl