5GYA
Crystal structure of ENZbleach xylanase V5N+V6N+K7R+K223R+K227R mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-23 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.501, 46.250, 78.204 |
| Unit cell angles | 79.57, 83.10, 66.25 |
Refinement procedure
| Resolution | 30.000 - 1.930 |
| R-factor | 0.1684 |
| Rwork | 0.166 |
| R-free | 0.21760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gv1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.375 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.000 |
| High resolution limit [Å] | 1.930 | 4.150 | 1.930 |
| Rmerge | 0.033 | 0.027 | 0.101 |
| Number of reflections | 37834 | ||
| <I/σ(I)> | 23.9 | ||
| Completeness [%] | 93.9 | 98.6 | 83.2 |
| Redundancy | 2.9 | 3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5 | 298 | PEG 6000, 0.1 M NaOAc, 0.2 M NaCl |
