5GY6
Ribonuclease from Hericium erinaceus (RNase He1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2016-03-09 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.701, 42.945, 60.183 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.958 - 1.500 |
R-factor | 0.1736 |
Rwork | 0.172 |
R-free | 0.19730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3who |
RMSD bond length | 0.011 |
RMSD bond angle | 1.240 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.960 |
High resolution limit [Å] | 1.500 |
Number of reflections | 13702 |
<I/σ(I)> | 12.31 |
Completeness [%] | 100.0 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 31% PEG-MME 550, 0.1M MES pH 7.0, 5mM ZnSO4 |