5GY3

The crystal structure of endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	53.570, 73.256, 79.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.245 - 1.770
R-factor	0.1646
Rwork	0.163
R-free	0.19520
RMSD bond length	0.007
RMSD bond angle	0.805
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.800
High resolution limit [Å]	1.770	4.800	1.770
Rmerge	0.081	0.055	0.208
Rmeas	0.092	0.063	0.236
Rpim	0.043	0.029	0.108
Total number of observations	130639
Number of reflections	30441
<I/σ(I)>	18.2
Completeness [%]	98.1	88.9	99.9
Redundancy	4.3	4.3	4.4
CC(1/2)		0.994	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	0.1M Glycine, pH 9, 30% PEG 8000, 0.5M KCl