5GXT
Crystal structure of PigG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.978 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 95.700, 60.000, 81.400 |
Unit cell angles | 90.00, 99.30, 90.00 |
Refinement procedure
Resolution | 47.221 - 2.245 |
R-factor | 0.2332 |
Rwork | 0.230 |
R-free | 0.29600 |
RMSD bond length | 0.014 |
RMSD bond angle | 0.925 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.221 | 2.420 |
High resolution limit [Å] | 2.245 | 2.300 |
Rmerge | 0.200 | 0.658 |
Number of reflections | 21702 | |
<I/σ(I)> | 4.7 | 2.1 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.8 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | MgCl2, Tris-HCl, pH 8.5, PEG 4000 |