5GXT
Crystal structure of PigG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.700, 60.000, 81.400 |
| Unit cell angles | 90.00, 99.30, 90.00 |
Refinement procedure
| Resolution | 47.221 - 2.245 |
| R-factor | 0.2332 |
| Rwork | 0.230 |
| R-free | 0.29600 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.925 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.221 | 2.420 |
| High resolution limit [Å] | 2.245 | 2.300 |
| Rmerge | 0.200 | 0.658 |
| Number of reflections | 21702 | |
| <I/σ(I)> | 4.7 | 2.1 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | MgCl2, Tris-HCl, pH 8.5, PEG 4000 |
