5GXI
Structure of the Gemin5 WD40 domain in complex with AAUUUUUGAG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-04-28 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.850, 124.520, 60.600 |
| Unit cell angles | 90.00, 116.77, 90.00 |
Refinement procedure
| Resolution | 28.900 - 1.850 |
| R-factor | 0.16614 |
| Rwork | 0.164 |
| R-free | 0.20738 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ow8 2hes 3dm0 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.894 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.920 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 65191 | |
| <I/σ(I)> | 14.9 | 1.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.3 | 7 |
| CC(1/2) | 0.998 | 0.592 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M sodium citrate, 0.2 M ammonium acetate, 15% PEG 4000 |
