5GXG
High-resolution crystal structure of the electron transfer complex of cytochrome p450cam with putidaredoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-24 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.490, 77.956, 59.968 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 59.660 - 1.700 |
| R-factor | 0.15788 |
| Rwork | 0.156 |
| R-free | 0.19158 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w9c |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.137 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.660 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.084 | |
| Number of reflections | 51169 | |
| <I/σ(I)> | 39.2 | |
| Completeness [%] | 99.6 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M HEPES, 0.1M Sodium Chloride, 1.6M Ammonium sulfate |
