5GXB
crystal structure of a LacY/Nanobody complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-01 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 96.543, 96.543, 145.025 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.342 - 3.300 |
R-factor | 0.34 |
Rwork | 0.340 |
R-free | 0.34650 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.844 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX ((dev_2405: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.342 | 3.600 |
High resolution limit [Å] | 3.300 | 3.500 |
Number of reflections | 10364 | |
<I/σ(I)> | 7.9 | 1.5 |
Completeness [%] | 97.1 | 96.4 |
Redundancy | 29.2 | 15.3 |
CC(1/2) | 0.988 | 0.485 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 100mM MES pH 6.0, 100mM (NH4)2C4H4O6, 43% PEG 400 |