5GX9
PYP mutant - E46Q
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | NUCLEAR REACTOR |
Source details | JRR-3M BEAMLINE 1G-B |
Synchrotron site | JRR-3M |
Beamline | 1G-B |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 2009-01-10 |
Detector | MACSCIENCE |
Wavelength(s) | 2.6 |
Spacegroup name | P 63 |
Unit cell lengths | 66.823, 66.823, 40.982 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.445 - 1.493 |
R-factor | 0.1623 |
Rwork | 0.158 |
R-free | 0.19960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ota |
RMSD bond length | 0.007 |
RMSD bond angle | 1.233 |
Refinement software | PHENIX ((1.12rc1_2815)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.550 |
High resolution limit [Å] | 1.493 | 1.493 |
Rmerge | 0.095 | 0.318 |
Number of reflections | 15995 | |
<I/σ(I)> | 7.78 | 2.4 |
Completeness [%] | 93.5 | 85.7 |
Redundancy | 2.2 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | AMMONIUM SULPHATE, SODIUM CHLORIDE, SODIUM DIDEUTERIUM PHOSPHATE, DISODIUM DEUTERIUM PHOSPHATE, pH 9.0, VAPOR DIFFUSION, HANGING DROP, MICROSEEDING, temperature 295K |