5GWN
Crystal structure of human RCC2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 94.447, 84.056, 60.671 |
Unit cell angles | 90.00, 101.57, 90.00 |
Refinement procedure
Resolution | 40.676 - 1.309 |
R-factor | 0.1721 |
Rwork | 0.171 |
R-free | 0.18890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a12 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.084 |
Data reduction software | DENZO |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.676 | 1.360 |
High resolution limit [Å] | 1.309 | 1.309 |
Number of reflections | 109282 | |
<I/σ(I)> | 9.3 | 2 |
Completeness [%] | 98.1 | 96.6 |
Redundancy | 12.8 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M BIS-TRIS 5.5, 0.2M (NH4)2SO4, 16% PEG 3350 |