5GWI
Structure of a Human topoisomerase IIbeta fragment in complex with DNA and E7873R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.788, 176.966, 94.528 |
| Unit cell angles | 90.00, 112.30, 90.00 |
Refinement procedure
| Resolution | 29.724 - 2.737 |
| R-factor | 0.215 |
| Rwork | 0.214 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qx3 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.509 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.724 | 2.840 |
| High resolution limit [Å] | 2.737 | 2.740 |
| Rpim | 0.290 | |
| Number of reflections | 62539 | |
| <I/σ(I)> | 13.6 | 3.03 |
| Completeness [%] | 98.2 | 96.7 |
| Redundancy | 3.6 | |
| CC(1/2) | 0.815 | 0.815 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | Magnesium Acetate, 2-(N-morpholino)ethanesulfonic, 2-methyl-2,4-pentanediol |
