5GWD
Structure of Myroilysin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-31 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.2816 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.223, 35.486, 93.757 |
Unit cell angles | 90.00, 101.41, 90.00 |
Refinement procedure
Resolution | 19.900 - 1.890 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.22400 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.847 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXCD |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.990 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.128 | 0.701 |
Number of reflections | 37660 | |
<I/σ(I)> | 11.9 | |
Completeness [%] | 98.6 | 91 |
Redundancy | 10.6 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | Sodium Citrate |