5GW1
Crystal structure of SNX16 PX-Coiled coil in space group P212121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.670, 133.060, 215.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.995 - 3.350 |
R-factor | 0.2586 |
Rwork | 0.257 |
R-free | 0.28630 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.468 |
Data reduction software | Aimless |
Data scaling software | Aimless (0.5.12) |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 61.910 | 61.910 | 3.530 |
High resolution limit [Å] | 3.350 | 10.590 | 3.350 |
Rmerge | 0.098 | 0.057 | 0.704 |
Rmeas | 0.112 | 0.066 | 0.799 |
Rpim | 0.053 | 0.032 | 0.371 |
Total number of observations | 139054 | 4270 | 20585 |
Number of reflections | 32016 | ||
<I/σ(I)> | 13.8 | 43.3 | 2.5 |
Completeness [%] | 99.7 | 96.2 | 99.9 |
Redundancy | 4.3 | 4 | 4.5 |
CC(1/2) | 0.994 | 0.986 | 0.683 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 277 | 31mM Citric, 69mM Bis-tris Propanol, 18% PEG 5000, 70mM NaF, 2mM TCEP |