5GVS
Crystal structure of the DDX41 DEAD domain in an apo open form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 91.188, 50.960, 202.599 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 49.410 - 2.200 |
| R-factor | 0.21071 |
| Rwork | 0.207 |
| R-free | 0.27487 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gvr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.696 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.410 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.202 | |
| Number of reflections | 36258 | |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 76.5 | |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | tri-ammonium citrate, PEG 3350 |
