5GVS

Crystal structure of the DDX41 DEAD domain in an apo open form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-19
Detector	RAYONIX MX225HE
Wavelength(s)	0.979
Spacegroup name	I 1 2 1
Unit cell lengths	91.188, 50.960, 202.599
Unit cell angles	90.00, 95.51, 90.00

Refinement procedure

Resolution	49.410 - 2.200
R-factor	0.21071
Rwork	0.207
R-free	0.27487
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gvr
RMSD bond length	0.013
RMSD bond angle	1.696
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.410	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.202
Number of reflections	36258
<I/σ(I)>	9.3
Completeness [%]	76.5
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	tri-ammonium citrate, PEG 3350