5GVM
Plasmodium vivax SHMT bound with PLP-glycine and GS557
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.858, 58.790, 234.062 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.240 |
| R-factor | 0.2458 |
| Rwork | 0.240 |
| R-free | 0.29710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tmr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.471 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.010 | 50.000 | 2.330 |
| High resolution limit [Å] | 2.240 | 4.850 | 2.250 |
| Rmerge | 0.026 | 0.022 | 0.137 |
| Number of reflections | 64408 | ||
| <I/σ(I)> | 28.2 | ||
| Completeness [%] | 96.2 | 92.3 | 84.3 |
| Redundancy | 3 | 3 | 2.1 |
| CC(1/2) | 0.998 | 0.975 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 298 | PEG4000, 0.06-0.12M NaCl, 0.1M Tris-HCl pH 8.5 |
