5GVE

Human TOP3B-TDRD3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-05-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	173.563, 173.563, 111.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.997 - 3.606
R-factor	0.2004
Rwork	0.198
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gvc 5gvd
RMSD bond length	0.004
RMSD bond angle	1.135
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.660
High resolution limit [Å]	3.600	3.600
Number of reflections	22224
<I/σ(I)>	11.2
Completeness [%]	99.9
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	50 mM Na-cacodylate, 10 mM MgSO4, 1.7 M Li2SO4