5GVB

SepB domain of human AND-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	F 4 3 2
Unit cell lengths	249.646, 249.646, 249.646
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.610 - 2.750
R-factor	0.214
Rwork	0.209
R-free	0.25400
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.709
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXDE
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.750	2.750
Rmerge	0.107	0.698
Number of reflections	34000
<I/σ(I)>	10.9
Completeness [%]	99.9	100
Redundancy	19.8	18.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	2.5M sodium formate pH7.0