5GVA

WD40 domain of human AND-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.91769
Spacegroup name	P 43 21 2
Unit cell lengths	79.895, 79.895, 163.744
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.148 - 1.851
R-factor	0.1709
Rwork	0.170
R-free	0.18840
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	1.075
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXDE
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.850	1.850
Number of reflections	45822
<I/σ(I)>	30.48
Completeness [%]	99.5	94.7
Redundancy	7.7	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	1.8M Ammonium citrate tribasic pH7.5