5GUY
Structural and functional study of chuY from E. coli CFT073 strain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97941, 0.97955, 0.97243 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.686, 67.836, 64.381 |
| Unit cell angles | 90.00, 90.35, 90.00 |
Refinement procedure
| Resolution | 34.254 - 2.399 |
| R-factor | 0.2135 |
| Rwork | 0.208 |
| R-free | 0.25900 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.088 | 0.619 |
| Number of reflections | 13433 | |
| <I/σ(I)> | 27.1 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.6 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 295 | 0.1M Bis-Tris pH 6.5, 50mM CaCl2, 30% PEG MME 550 |
