5GUS
Crystal structure of ASCH domain from Zymomonas mobilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 51.999, 51.999, 207.372 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.000 - 1.951 |
| R-factor | 0.1542 |
| Rwork | 0.152 |
| R-free | 0.19700 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.810 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.980 |
| High resolution limit [Å] | 1.951 | 1.951 |
| Number of reflections | 24746 | |
| <I/σ(I)> | 22.5 | 5 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 8.5 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 40% (w/v) polyethylene glycol 400, 0.1M cacodylic acid (pH 6.5), 0.2M lithium sulfate |
