5GUQ
Crystal structure of ASCH from Zymomonas mobilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-31 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.910, 52.859, 88.675 |
| Unit cell angles | 90.00, 111.56, 90.00 |
Refinement procedure
| Resolution | 30.592 - 1.697 |
| R-factor | 0.1994 |
| Rwork | 0.198 |
| R-free | 0.23540 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.264 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.600 | 1.760 |
| High resolution limit [Å] | 1.697 | 1.700 |
| Number of reflections | 68764 | |
| <I/σ(I)> | 17.6 | 2.5 |
| Completeness [%] | 98.7 | 99.1 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% (w/v) PEG 3350, 0.1M MES (pH 6.5) |
