5GUJ
Crystal structure of the Bacillus subtilis DnaG RNA Polymerase Domain, natural degradation of full length DnaG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 61 |
| Unit cell lengths | 117.114, 117.114, 48.858 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.278 - 2.500 |
| R-factor | 0.1908 |
| Rwork | 0.189 |
| R-free | 0.23880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e2k |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.897 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.278 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.123 | 0.429 |
| Number of reflections | 13480 | |
| <I/σ(I)> | 20.7 | 6.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.2 M Sodium citrate tribasic dehydrate, 0.1M Tris hydrochloride (pH 8.5) and 30% (w/v) polyethylene glycol 400 |
