5GUC
Crystal structure of CotB2 (apo form) from Streptomyces melanosporofaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-12-22 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.274, 101.182, 106.991 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.320 - 1.800 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.20100 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.297 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.320 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.084 | 0.515 |
| Number of reflections | 62332 | |
| <I/σ(I)> | 29 | |
| Completeness [%] | 99.5 | 98.1 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M HEPES-NAOH (PH8.0), 1.4M AMMONIUM FORMATE |
