5GU6
Crystal structure of Human ERp44 form I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-18 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 81.630, 81.630, 139.910 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.607 - 2.000 |
R-factor | 0.2057 |
Rwork | 0.204 |
R-free | 0.24050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2r2j |
RMSD bond length | 0.004 |
RMSD bond angle | 0.665 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.610 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.075 | 1.125 |
Number of reflections | 36963 | |
<I/σ(I)> | 22.5 | 2.2 |
Completeness [%] | 99.5 | 99.12 |
Redundancy | 18.2 | 11.14 |
CC(1/2) | 0.998 | 0.759 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 277 | Polyethylene glycol (PEG) 8000 |