5GU5
Crystal structure of p24gamma2 GOLD domain determined by sulfur-SAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-06-12 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 79.410, 79.410, 125.948 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.833 - 2.800 |
R-factor | 0.2261 |
Rwork | 0.224 |
R-free | 0.27390 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.730 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX ((1.10_2155: 000)) |
Refinement software | PHENIX ((1.10_2155: 000)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.762 | |
Number of reflections | 6265 | |
<I/σ(I)> | 72.1 | 4.5 |
Completeness [%] | 99.0 | 100 |
Redundancy | 12.8 | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 60% (v/v) Tascimate (pH 8.0) |