5GU2
Crystal structure of Au(M).CL-apo-E45C/R52C-rHLFr
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.03895 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 181.079, 181.079, 181.079 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.360 - 2.120 |
R-factor | 0.18755 |
Rwork | 0.185 |
R-free | 0.22863 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dat |
RMSD bond length | 0.020 |
RMSD bond angle | 1.845 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.120 | |
Number of reflections | 14946 | |
<I/σ(I)> | 60.7 | 11.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.5 | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium Sulphate, Cadmium Sulphate |