5GTX
Crystal structure of mutated buckwheat glutaredoxin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9776 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 53.251, 62.185, 143.407 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.447 - 2.280 |
R-factor | 0.2145 |
Rwork | 0.213 |
R-free | 0.24220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kqa |
RMSD bond length | 0.007 |
RMSD bond angle | 0.897 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX (1.10.1-2155) |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.280 | 2.280 |
Number of reflections | 11059 | |
<I/σ(I)> | 29.8 | 3.67 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.7 | 12.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 300 | 100mM CH3COONa pH4.5 and 23%(w/v) PEG3350 |