5GTU
Structural and mechanistic insights into regulation of the retromer coat by TBC1d5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2014-03-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.881, 63.832, 78.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.010 - 1.500 |
R-factor | 0.14089 |
Rwork | 0.139 |
R-free | 0.18555 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z2x |
RMSD bond length | 0.026 |
RMSD bond angle | 2.288 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 1.500 |
Number of reflections | 33729 |
<I/σ(I)> | 38 |
Completeness [%] | 98.1 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 289 | 0.1M Bis-Tris, pH6.0, 2% Tacsimate, pH 6.0, 15~20% PEG3350 |