5GSY
Kinesin-8 motor, KIF19A, in the nucleotide-free state complexed with GDP-taxol microtubule
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TECNAI ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 7 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
