5GSV
Mouse MHC class I H-2Kd with a MERS-CoV-derived peptide 142-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-07-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.31573 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.450, 45.853, 71.644 |
| Unit cell angles | 90.00, 108.72, 90.00 |
Refinement procedure
| Resolution | 37.992 - 1.996 |
| R-factor | 0.2029 |
| Rwork | 0.200 |
| R-free | 0.25240 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.911 |
| Data reduction software | CNS |
| Data scaling software | CNS |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.996 |
| <I/σ(I)> | 1.5 |
| Completeness [%] | 96.8 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 0.1M Sodium citrate tribasic dihydrate pH 5.0, 10% w/v Polyethylene glycol 6000 |
