5GSU

Crystal structure of nucleosome core particle consisting of human testis-specific histone variants, Th2A and Th2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-28
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	107.095, 109.740, 182.445
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.330 - 3.100
R-factor	0.2
Rwork	0.197
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3x1u
RMSD bond length	0.012
RMSD bond angle	1.350
Data scaling software	HKL-2000 (Scala)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.150
High resolution limit [Å]	3.100	3.100
Rmerge	0.129
Number of reflections	38482
<I/σ(I)>	20
Completeness [%]	96.8	98.9
Redundancy	12.3	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	20mM Pottasium Cacodylate pH 6.0, 60-70mM KCl, 70-90mM MnCl2