5GSM
Glycoside hydrolase B with product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2016-05-26 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.585, 119.991, 85.902 |
| Unit cell angles | 90.00, 98.66, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.270 |
| R-factor | 0.13251 |
| Rwork | 0.130 |
| R-free | 0.17543 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.444 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.270 |
| Number of reflections | 370867 |
| <I/σ(I)> | 20.8 |
| Completeness [%] | 92.5 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM PCB buffer pH7.0, 25% (w/v) PEG 1500, 50mM GlcN |
