5GSL
Glycoside hydrolase A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2014-11-25 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 146.843, 148.767, 165.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
R-factor | 0.22076 |
Rwork | 0.217 |
R-free | 0.28778 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.511 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.600 |
Number of reflections | 51643 |
<I/σ(I)> | 10.3 |
Completeness [%] | 92.2 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM Tris-HCl pH8.5, 20% (w/v) PEG 1000 |